FIGURE 2.

The MBD family of proteins. All MBD family proteins contain a highly conserved MBD (blue box) in addition to other functional domains. The MBD of MBD3 has a point mutation (^∗) that abolishes methyl-CpG binding. MBD5 and MBD6 do not specifically bind methyl-CpG (lighter blue box). MeCP2, MBD1, and MBD2 contain C-terminal transcriptional repression domains (TRD) that interact with co-repressor protein complexes (orange box). MBD1 has CxxC-type zinc finger domains which mediate DNA binding. MBD2 has an N-terminal glycine/arginine rich domain (yellow box) and a C-terminal coiled-coil domain, which is also found in MBD3 (green box). The functions of MBD4 are mediated through a C-terminal glycosylase domain (red box). Both MBD5 and MBD6 contain proline rich domains (tan box) while MBD5 has a PWWP motif (purple box) that binds methylated histones.