FIGURE 6.

DNA binding dynamics of MBD2 isoforms depend on post-translational modifications. (A) MBD2a and NuRD binding to methylated sites results in nucleosome remodeling (thick arrows) and deacetylation (red triangles), producing transcriptional silencing. (B) Post-translational methylation of the N-terminal glycine/arginine rich region of MBD2a by PRMT1/5 reduces the affinity of MBD2a for NuRD and methylated DNA and may not produce transcriptional repression. (C) MBD2c does not interact with the NuRD complex but does bind methylated DNA. It is not fully understood how this binding may affect transcriptional regulation. (D) MBD2c has the N-terminal G/R rich domain, but it is not clear if it is also methylated by PRMT1/5 and if this modification would affect DNA binding similarly to MBD2a.