Fig. 5.

STEAP1 vaccination reduces tumour burden and increases T-cell infiltration in the prostate in spontaneous prostate cancer model. A 3-week interval vaccination was initiated on 6- to 8-week-old TRAMP mice using 10⁸ IU of ChAdOx1.STEAP1 and 10⁷ PFU of MVA.STEAP1. Immunised and control unvaccinated TRAMP mice were sacrificed at 25–27 weeks of age, and genitourinary tracts (GUTs) were dissected, weighed and embedded in paraffin. Representative data of 3 biological replicate experiments are shown. a Ratio of GUT wet weight to whole body weight in control and immunised TRAMP mice at 25–27 weeks of age. Bars represent median. b, c Immunohistochemistry staining of GUTs in STEAP1 vaccinated and control mice. Quantitative analysis and representative pictures of Ki-67 positive staining (b) and CD3 expression (c) of FFPE prostate tissue sections. Bars represent median. Brown regions indicate immunoreactivity; counterstain is haematoxylin (×200). Scale bar 100 μm