Figure 3.

White cells are more virulent than opaque cells at higher temperatures. (A) Wild type zebrafish larvae were infected with C. albicans white cells (CAY4975), opaque cells (CAY4986), or mock infected. Embryos were kept at the indicated temperature after infection and monitored daily for survival. Data are a compilation of 11, 8, and 7 independent experiments for 25, 30, and 33°C, respectively. N = 14–235 fish/group for each inoculation interval. Statistically significant differences were determined using a Log rank (Mantel-Cox) test. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001. (B) Median survival (time to 50% survival or endpoint of experiment) was compared for fish infected with the indicated inoculums of C. albicans white or opaque cells housed at 25, 30, and 33°C.