Figure 6.

Analysis of the diffusion coefficients of mMaple3 in 87 E. coli cells measured with STICS. (A) The measured M_C may be converted to unbiased M_C based on the simulations in Fig. 3A, which are used here to create a lookup table. The overlapping colored lines represent the same experimental parameter sets as in Fig. 3A (Table S1). The black line indicates 1:1 correspondence (no bias). (B) Histogram of the unbiased average mMaple3 diffusion coefficient measured in each of 87 different E. coli cells as estimated by the STICS method. Between 5 and 15 mMaple3 fluorescent proteins are photoswitched one at a time in each cell, and the biased measurements are corrected by cubic interpolation of the curve in (A). To see this figure in color, go online.