Figure 2.
Illustration of the reference FRAP experiment performed on single spheres. (A) Confocal laser scanning micrograph of single sphere with fluorescently labeled lipid membrane before bleaching. (B) Fluorescence micrograph after bleaching half of the sphere vertically; bleaching area highlighted as black half-circle. (C) Recovery of fluorescence intensity within bleached area. Overall intensity lowered. (D) Schematic representation of the FRAP experiment with x0 as a variable bleaching position. All fluorescence micrographs are in gray scale with inverted colors. Scale bar, 5 μm. To see this figure in color, go online.