Figure 4.

(A) Example FRAP experiment performed on a pair of fused membrane-coated silica beads. Fluorescence recovery within the bleached area (circles) alongside the intensity of a single reference bead (squares) to correct for general bleaching and the background (diamonds) is shown. (B) Normalized fluorescence recovery $f_n\left(t\right)$ (circles), and the fluorescence depletion $F_n\left(t\right)$ calculated (squares). To see this figure in color, go online.