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. 2016 May 26;17:52. doi: 10.1186/s12882-016-0265-z

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Calcium deposition a Ca²⁺ deposition in VSMC derived from Gas6^-/- and WT mice after 168 h (h) of exposure to phosphate and calcium-enriched cell culture medium. b TUNEL-positive VSMC of Gas6 and WT mice after exposure to phosphate and calcium-enriched cell culture medium. VSMC (Vascular Smooth Muscle Cells), WT (Wildtype); mean ± SD; *: the increase in apoptosis was significant for WT cells after 120 h and 168 h compared to 0 h (p < 0.05); ** the increase in calcification was highly significant for WT cells after 168 h compared to 0 h (p < 0.001)