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. 2016 May 25;11:45. doi: 10.1186/s13000-016-0495-1

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© Ambrosio et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Immunocytochemical analysis of HIF-1α in LNCaP and PC-3 cells, under normoxia and hypoxia. No staining or mild cytoplasmic positivity for HIF-1α is detected in LNCaP (a) and PC-3 (b) cells, under normoxia. Under hypoxia, cytoplasmic positivity becomes stronger, both in LNCaP (c) and PC-3 cells (d). Nuclear translocation of the protein is also evident (c-d) (a-d, O.M.: 400x)