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. 2016 May 26;16:121. doi: 10.1186/s12870-016-0812-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Induction, verification, and growth polymorphism observation of four different types of hairy root lines. (a) Structure and restriction maps of the transformation vectors pBI121-eGFP (EV), pBI121-LeEIL-1-RNAi (Ei), and pBI121-LeEIL-1-Overexpression (EO); (b) Growth polymorphism of the induced L. erythrorhizon hairy roots by using the method of pricking nodes with a needle. Differentiated callus, leaf (c), and regenerated shoots (d) from the Ei hairy root lines on the stock culture medium; (e) PCR analysis of the rolC gene in hairy roots. The untransformed L. erythrorhizon seedling was used as the negative control (CK)