Figure 6.

(A) The HEK293 cell was transfected with pcDNA-tcasp-1-HA and/or pcDNA-tIL-1β-Flag. Culture supernatant samples and cell lysates were collected 36 h post-transfection and detected by Western blotting; (B) HEK293 cells were co-transfected with wild-type pcDNA-tIL-1β-Flag/mutant plasmids and pcDNA-tcasp-1-HA. Whole-cell lysates were subjected to Western blotting for the target proteins; (C) Expression of truncated tIL-1β from D130 to C-terminus in Sf9 cells; (D) The mRNA transcripts from stimulated PBMo were normalized to the non-stimulated group and results are expressed as mean ± SEM of three independent experiments.