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. 2016 May 26;17:403. doi: 10.1186/s12864-016-2745-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Ion Proton RNA-Seq library preparation workflow. a Life Technologies’ protocol: mRNAs were fragmented by RNaseIII using the Ion Total RNA-Seq Kit V2, only the sense RNA strands (mRNA) were sequenced. b BGI’s protocol: based on chemical (heat) fragmentation. Additional size selection steps were introduced after adaptor ligation