Figure 6.

Increase of WNT/beta-catenin activity in HCT-116 cells results in higher levels of ERK1/2 activation and decreased ability for clonal growth at 42 °C. (A,C) WNT/beta-catenin transcriptional activity was measured as in Figure 5 after exposure of the cells to mock treatment (M), 5 μM of the GSK-beta kinase inhibitor (GSKi) TWS119, or 0.5 μM AZD6244 (Ei) for 16 h. Three experiments with duplicate samples per treatment and condition (temperature) were performed; (B,D) Clonal growth analyses were performed as in Figure 1 by exposing the cells for 24 h to mock treatment or 5 μM TWS119 (GSKi); (E) Representative Western blot analyses with total cell lysates of HCT-116 cells exposed for 16 h to 42 °C and 5 or 10 μM TWS119 (GSKi) and 20 mM lithium chloride.