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. 2016 May 26;6:26909. doi: 10.1038/srep26909

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(A) The tight junction protein Claudin-5 immunostaining. (B) The tight junction protein Occludin immunostaining. (C) Western blot analysis to determine ZO-1 protein expression in the third month control retina, diabetic retina, and the retina of Niaspan-treated rats. Lane loading was normalized by reblotting with a monoclonal anti-β-actin antibody. Relative expression levels of ZO-1 (n = 4/group) are present. (E) RT-PCR experiments to determine Claudin-5, Occludin, ZO-1 expression in the retina. *P < 0.05 compared with nondiabetic retina (CON). ^#P < 0.05 compared with diabetic retina (DR).