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. 2016 May 26;6:26581. doi: 10.1038/srep26581

Figure 7. Production of TFV was more severely inhibited by co-depletion of Alix, Tsg101, and Nedd4.1.

Figure 7

(A) Co-depletion of 100 nM siAlix and 100 nM siTsg101 or 100 nM siGFP were transfected into cells 6 h prior to infection with TFV for 1 h, and then cells were cultured for 66 h. Cells and supernatant were lysed to extract DNA. Samples were then subjected to qPCR using MCP primers to quantify TFV genomic concentration. (B) Co-depletion of 100 nM siAlix and 100 nM siNedd4.1 compared with 100 nM siGFP. (C) Co-depletion of 100 nM siTsg101 and 100 nM siNedd4.1 compared with 100 nM siGFP. (D) Co-depletion of 100 nM siTsg101, 100 nM siNedd4.1, and 100 nM siAlix compared with 100 nM siGFP. Statistical analysis was performed using Student’s t-test (0.05 < *p ≤ 0.1, 0.01 < **p ≤ 0.05, 0.01 ≤ ***p).