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. 2016 May 26;14:148. doi: 10.1186/s12967-016-0904-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — SP dependent up regulation of cytokine and chemokine production by human PBMC* is inhibited by aprepitant. *Freshly isolated PBMC from normal donors were incubated for 24 h with SP and/or Aprepitant (10 µM each) as indicated. Control cultures were either left untreated of incubated with DMSO (vehicle for aprepitant). Pro-inflammatory markers were measured in supernatants using MILLIPORE Human Cytokine/Chemokine Magnetic Bead Panel. Results are presented as mean ± SD for three independent experiments. *Statistically significant difference from control (p < 0.05)