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. 2016 May 26;6:26559. doi: 10.1038/srep26559

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) AxonJ generated heat map of a whole optic nerve section showing the subimages that were sampled for manual counting by human experts indicated by boxes. (b) Density heat map for strain matched wt mice (image set C0), (c) density heat map for nee mice (image set C1), demonstrating the regional variation in density as well as optic nerve cross-sectional area. The heat map shows denser areas of axons as hotter, while colder regions represent less dense regions of axons. Whole-nerve axon count and optic nerve area (μm²) are provided for each cross section. Scale bar (axons/100 μm²).