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. 2016 May 26;6:26807. doi: 10.1038/srep26807

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Copyright © 2016, Macmillan Publishers Limited

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Effects of U50,488H on Akt/eNOS phosphorylation in the absence and presence of nor-BNI and PI3K/Akt/eNOS inhibitors (a) as well as siRNAs targeting κ-OR and Akt (b) in vitro (n = 5). Values are mean ± SEM. Con: normal medium group, Con + U: normal medium + U50,488H group, P: palmitate-added medium group, P + U: palmitate-added medium + U50,488H group, P + U + N: palmitate-added medium + U50,488H + nor-BNI group, P + U + LY: palmitate-added medium + U50,488H + LY294002 group, P + U + MK: palmitate-added medium + U50,488H + MK2206-HCl group, P + U + L: palmitate-added medium + U50,488H + L-NAME group. P + U + NC: palmitate-added medium + U50,488H + non-targeting siRNA group, P + U + κ2: palmitate-added medium + U50,488H + κ-OR siRNA2 group, P + U + κ3: palmitate-added medium + U50,488H + κ-OR siRNA3 group, P + U + A2: palmitate-added medium + U50,488H + Akt siRNA2 group, P + U + A3: palmitate-added medium + U50,488H + Akt siRNA3 group. *P < 0.05, **P < 0.01 vs. Con, ^#P < 0.05 vs. P, ^##P < 0.01 vs. P, ^$$P < 0.01 vs. P + U (a), ^$P < 0.05 vs. P + U + NC (b).