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. 2016 May 26;6:26446. doi: 10.1038/srep26446

Figure 3. Long-term recombinant protein expression stability in transfected CHO cells.

Figure 3

Stably transfected CHO cells were selected after transfection by the addition of G418. The cells were split in two and further cultured either in the presence or the absence of G418 before flow cytometric analysis. The eGFP expression retention was calculated as the ratio of the MFI at the end of stability testing to the MFI at the start of stability testing. Relative changes in eGFP expression level at different days post-transfection in the presence (A) or absence (B) of selection pressure. Retention of eGFP expression levels in cells transfected with SV40, RSV, and CMV promoter-containing vectors (C; n = 3).