Figure 4. Fluorescence microscopy of eGFP gene in transfected CHO cells grown in the presence or absence of G418 selection pressure.
CHO cells transfected with (A) the CMV promoter-containing vector at day 30 post-transfection in the presence of selection pressure; (B) the CMV promoter-containing vector at day 78 post-transfection in the presence of selection pressure; (C) the SV40 promoter-containing vector at day 30 post-transfection in the presence of selection pressure; (D) the SV40 promoter-containing vector at day 78 post-transfection in the presence of selection pressure; (E) the CMV promoter-containing vector at day 30 post-transfection in the absence of selection pressure; (F) the CMV promoter-containing vector at day 78 post-transfection in the absence of selection pressure; (G) the SV40 promoter-containing vector at day 30 post-transfection in the absence of selection pressure; (H) the SV40 promoter-containing vector at day 78 post-transfection in the absence of selection pressure.