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. 2016 May 26;6:26419. doi: 10.1038/srep26419

Figure 5. GR regulation of mitochondrial function.

Figure 5

(A) A549 cells were transfected with 1 μg of either HaloTag GRα or GRγ, then fixed and immunolabelled with antibodies for HaloTag (green) or mitochondrial HSP70 (red). Higher magnification images of boxed regions are shown inset. Representative images are shown. (B) Mitochondrial morphology (perimeter, area, circularity) was quantified where each individual data point represents an average across a single cell. Data are presented as the mean ± SD. (C) HEK-FlpGRα and HEK-FlpGRγ cells were treated with 100 nM dexamethasone overnight, then incubated with Mito Tracker Green for 1 hr, and analysed by FACS to measure mitochondrial mass. Shown are representative histograms and quantification of mean fluorescence intensity (MFI) for Mitotracker Green staining. Samples compared with one-way ANOVA, and data presented as the mean ± SD of three independent experiments (*p < 0.05). (D) HEK-FlpGRα and HEK-FlpGRγ cells were treated with 100 nM dexamethasone overnight, then mitochondrial function measured on a Seahorse XFe96 analyser. Samples compared with one-way ANOVA, and data presented as the mean ± SD of three independent experiments (*p < 0.05). OCR oxygen consumption rate.