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. 2016 May 26;17:406. doi: 10.1186/s12864-016-2741-z

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — pCAM48 map. pCAM48 is a temperature-sensitive shuttle vector for delivery of a Himar1 mini-transposon, flanked by inverted repeat (IR) sequences modified to contain MmeI restriction enzyme sites. An erythromycin resistance marker (Erm^r) is included within the transposon, as is the R6kγ origin of replication (Ori R6kγ), which can be used for plasmid rescue (not employed in this study). Outside of the mini-transposon, the vector contains a ColE1 origin of replication, the Gram-positive temperature-sensitive replicase RepA TS, in which the single-bp deletion present in pCAM45 has been repaired, and a kanamycin resistance marker (Kan^r). The C9 Himar1 transposase gene is under the control of the S. pyogenes M1 gyrA promoter. Restriction enzyme sites used in development and analysis of pCAM48 are labeled