Fig. 4.

Effect of α7 nAChR activation on miniature EPSCs and IPSCs. Miniature IPSC (mIPSC) and EPSC (mEPSC) frequencies were measured in layer V pyramidal neurons of the prelimbic cortex in the presence of tetrodotoxin (1 μM) to block axonal conduction. Cumulative distribution and summary histograms (inserts) of mIPSC (A,B) and mEPSC (C,D) frequencies in the presence or absence of PNU-120596 (10 μM) alone (‘PNU 1’; n = 11 (9)) (A,C) or PNU-120596 (10 μM) co-applied with PNU-282987 (300 nM) (‘PNU 1 + PNU 2’; n = 5 (4)) (B), or PNU-282987 (30 nM) (‘PNU 1 + PNU 2’; n = 3 (3)) (D). Representative traces are shown beneath each panel. * significantly different from control, p ≤ 0.001, K–S test based on corresponding cumulative frequency plot. Scale bars: 30 pA and 0.5 s.