Fig. 5.

Enhancing endogenous acetylcholine selectively potentiates excitatory signalling. Spontaneous EPSCs (sEPSCs) and IPSCs (sIPSCs) were recorded from layer V pyramidal neurons in the presence and absence of donepezil (10 μM) to block acetylcholinesterase activity. A. Cumulative inter-event interval distributions and summary histogram of sEPSC and sIPSC frequencies confirm that donepezil had no effect on either sEPSC or sIPSC frequency; n = 8 (5). B,C Cumulative inter-event interval distributions and summary histogram of sEPSCs (B) and sIPSCs (C) in the presence and absence of PNU-120596 (10 μM), donepezil (10 μM), and MLA (100 nM), n = 6 (6). * significantly different, p ≤ 0.001, K–S test based on corresponding cumulative frequency plot.