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. 2016 Apr 25;17(5):615. doi: 10.3390/ijms17050615

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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TNFSF15 prevents the decrease in claudin-5, occludin, and ZO-1 protein levels in the rat retinas induced by diabetes. (A) Typical images of tight junction protein immunostaining in the retina. Arrows indicate claudin-5 (upper panel), occluding (middle panel) or ZO-1 (lower panel) expression in the retina. Magnification 400×; (B) A representative Western blot is shown; (C) Image of a Western blot analysis is shown: the data are shown as a percentage of the control and are presented as the mean ± SD of eight eyes. ANOVA (one-way) followed by the LSD test. * p < 0.001, significantly different from the control group; ** p < 0.001, significantly different from the DM group.