Figure 7.

Cytotoxic effect of rMbovNase on BoMac cells. (A) BoMac cells treated with rMbovNase or rMbovNase^Δ181–342 proteins for 24 h at 37 °C, and cell viability was determined by MTT assay. Flow cytometry assay on BoMac cells treated with rMbovNase (B) and rMbovNase^Δ181–342 (C) protein for 24 h and stained with Annexin V and propidium iodide (PI). PBS treatment was taken as a negative control (NC); apoptosis inducer from the commercial kit was a positive control (PC). * and *** represent p < 0.05 and 0.001 respectively.