Table 1.
Oligonucleotide primers for PCR used in this study.
| Names | Primer Sequences (5′-3′) | Positions |
|---|---|---|
| MBOV_RS02825 | ||
| 580F1 | CGGGGTACCGAAAATGGCACAATTAAG | 79→97 |
| 580R1 | AGTTTTTTCTCCCAGCCATCTACTAACTC | 316←345 |
| 580F2 | GTTAGTAGATGGCTGGGAGAAAAAACTTAG | 317→347 |
| 580R2 | AATATTTAGCCTTCAATTTGTCCCAATCTATG | 437←469 |
| 580F3 | CTAAATATTTTGATGCAGAAATAGTTAAATGGAGCG | 460→496 |
| 580R3 | GGCATAATGCTCCCAGTAAACTGGATTTTT | 855←885 |
| 580F4 | AATCCAGTTTACTGGGAGCATTATGCC | 855→885 |
| 580R4 | TAAATGTTAGATTGAATCCAGTATGGC | 956←983 |
| 580F5 | GCCATACTGGATTCAATCTAACATTTA | 956→983 |
| 580R5 | AGTGTTTATCTAGCAATGTCCATTTTC | 1000←1027 |
| 580F6 | TGGAAAATGGACATTGCTAGATAAACA | 998→1025 |
| 580R6 | CGCGGATCCTTATTTATTTTTGTATGAATC | 1071←1092 |
| MBOV_RS02825ΔTNASE_3 | ||
| 580F1 | CGGGGTACCGAAAATGGCACAATTAAG | 79→97 |
| 580UR | TGGCAATGCAAATTTAGCCTTCAATTT | 530←543, 1027←1040 |
| 580DF | AAATTGAAGGCTAAATTTGCATTGCCA | 530→543, 1027→1040 |
| 580R6 | CGCGGATCCTTATTTATTTTTGTATGAATC | 1071←1092 |
The underlined sequences were sites for restrictive digestion. The bold indicates TGA to TGG change to permit tryptophan expression in E. coli. The arrows showed nucleotide position within MBOV_RS02825 coding open reading frame (ORF).