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Double immunofluorescence staining of LCN2 and myeloperoxidase (MPO)-positive cells in representative sections of lung tissue for control rats (cont) and 3, 12 and 24 h after irradiation. Cryosections were stained with anti-LCN2 (red) and anti-MPO (green), followed by fluorescence immunodetection. Counterstaining of the nuclei was done with DAPI (blue) (original magnification 200×, scale bar = 20 μm).
