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. 2016 Apr 29;17(5):648. doi: 10.3390/ijms17050648

Figure 3.

Figure 3

Knockdown of Lsd1 induces cell death in wing imaginal discs. The atrophied wing phenotype of Lsd1-knockdown flies was rescued by P35 (B,B’) and DIAP1 (C,C’); (A,A’) MS1096-GAL4 > UAS-Lsd1IR; (B,B’) MS1096-GAL4/+; UAS-Lsd1IR/+; UAS-P35/+ (MS1096-GAL4 > UAS-Lsd1IR/UAS-P35); (C,C’) MS1096-GAL4/+; UAS-Lsd1IR/+; UAS-DIAP1/+ (MS1096-GAL4 > UAS-Lsd1IR/UAS-DIAP1); (D–F) immunostaining of wing imaginal discs with anti-active caspase-3 antibody; Control lines, MS1096-GAL4 (D) and MS1096-GAL4 > UAS-GFPIR (E); showed few cell death signals, while Lsd1-knockdown line MS1096-GAL4 > UAS-Lsd1IR (F) showed increased cell death signals via apoptosis; (G) average number of apoptotic cells in the wing pouch (n = 10); *, p < 0.05. Data are expressed as the mean ± S.D; (H,I) autophagy was determined by LysoTracker staining; (H) the control fly MS1096-GAL4; (I) Lsd1-knockdown fly MS1096-GAL4 > UAS-Lsd1IR. The circle indicates the wing pouch of the wing disc. The flies were reared at 25 °C.