Figure 4.

Knockdown of Lsd1 induces ectopic ROS in wing imaginal discs. Wing discs of third-instar larvae were incubated with substrate CM-H₂DCFDA. The control lines MS1096-GAL4 (A) and MS1096-GAL4 > UAS-GFPIR (B) showed an unclear ROS signal in the wing disc (marked by the circle), while the Lsd1-knockdown line (MS1096-GAL4 > UAS-Lsd1IR) showed an increased ROS signal (C); (D) Quantification of fluorescent ROS signals in the wing pouch region. Mean intensities with standard deviation from six imaginal discs are shown; * p < 0.05, Student’s t test.