Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 29;17(5):648. doi: 10.3390/ijms17050648

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Knockdown of Lsd1 induces mitochondrial expansion and defects in ATP and free fatty acids (FFA) production. (A,B) The green fluorescent protein (GFP) signal expressed in mitochondria of wing disc cells were observed under BX-50 fluorescence microscopy. The control flies MS1096-GAL4/+; +; UAS-MitoGFP/+ (MS1096-GAL4 > UAS-MitoGFP) showed normal mitochondrial matrix (A); while the Lsd1-knockdown flies MS1096-GAL4/+; UAS-Lsd1IR/+; UAS-MitoGFP/+ (MS1096-GAL4 > UAS-Lsd1IR/UAS-MitoGFP) appeared to exhibit mitochondrial expansion (B); Relative amounts of ATP (C) and FFA (D) of Lsd1-knockdown flies yw; Fb-GAL4/UAS-Lsd1IR; + (Fb-GAL4 > UAS-Lsd1IR). ATP and FFA amounts in whole bodies of third instar larvae were normalized to body weight. The experiments were repeated at least three times. * p < 0.05, ** p < 0.001, Student’s t test. The flies were reared at 25 °C.