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CCND1 and CCNE1 were reduced by REST knockdown. (A,B) The mRNA levels of two genes involved in G1–S cell cycle transition, CCND1 and CCNE1 were analyzed by real-time PCR; (C) The expression of CCND1 and CCNE1 was verified by Western blotting; (D,E) The quantifications of Western blotting were applied with Image J. * p < 0.05 compared with NC group.
