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. 2016 May 3;17(5):664. doi: 10.3390/ijms17050664

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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REST silencing elevated genes involved in cytostasis and migration inhibition. (A) The mRNA level of potential REST-regulated genes involved in cytostasis and migration inhibition were analyzed by real-time PCR, and GAPDH was used as an internal control; (B,C) ChIP (chromatin immunoprecipitation) assays were performed to pull down REST-DNA complexes from U-87 cells using anti-REST antibodies, and fold enrichment of BBC3 and DAXX was determined by real-time PCR; (D) SYN1 was used as a positive control; (E,F) The protein level of BBC3 and DAXX were verified by Western blotting; (G) The interactome was generated from the identified upregulated genes with the help of STRING database, using medium confidence (0.400) parameter, in order to identify highly-possible connections. * p < 0.05 compared with NC group.

REST silencing elevated genes involved in cytostasis and migration inhibition. (A) The mRNA level of potential REST-regulated genes involved in cytostasis and migration inhibition were analyzed by real-time PCR, and GAPDH was used as an internal control; (B,C) ChIP (chromatin immunoprecipitation) assays were performed to pull down REST-DNA complexes from U-87 cells using anti-REST antibodies, and fold enrichment of BBC3 and DAXX was determined by real-time PCR; (D) SYN1 was used as a positive control; (E,F) The protein level of BBC3 and DAXX were verified by Western blotting; (G) The interactome was generated from the identified upregulated genes with the help of STRING database, using medium confidence (0.400) parameter, in order to identify highly-possible connections. * p < 0.05 compared with NC group.