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Analysis of the purified laccase by PAGE (lanes a and b) and SDS-PAGE (lane c). Lane a, laccase activity after PAGE, using 1 mmol·L−1 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as substrate; lanes b and c, protein staining. Total protein loading was 25 µg per lane. M, molecular mass markers.
