Figure 5.

Effects of the reaction time and the laccase activity load on the decolorization of BPB, RB4 and RBBR by the pure Lacps1 (a,c), or the crude culture extract from P. sanguineus RP15 (b,d), at 40 °C. Dyes final concentrations were 100 mg·L⁻¹ (RBBR and RB4) or 25 mg·L⁻¹ (BPB), and the reactions were carried out in McIlvaine buffer, pH 4.5. (a,b). The enzymatic load was 5 U·mL⁻¹ (c,d); the reaction time was 120 min. Insets: Effects of the reaction time (a,b), and the laccase activity load (c,d), on dye decolorization by the crude extract (b,d), or the pure Lacps1 (a,c), at 25 °C. Data are presented as means ± SD from triplicate experiments (n = 3) performed with three separate preparations of crude extract or pure Lacps1 (error bars are not evident, as they lie within the area of the symbol). Symbols: BPB (■), RBBR (●) and RB4 (□).