Table 1.
Effect of different carbon sources and supplementary carbon and nitrogen sources on the production of laccases by P. sanguineus RP15.
| Carbon Source | U·g−1 | U·mL−1 | |
|---|---|---|---|
| Wheat bran | 29.8 ± 1.9 | 5.9 ± 0.4 | |
| Steam-exploded sugarcane bagasse | ND a | ND | |
| Raw sugarcane bagasse | ND | ND | |
| Peanut hull | 2.1 ± 0.2 | 0.40 ± 0.03 | |
| Rice husk | 1.9 ± 0.1 | 0.40 ± 0.02 | |
| Milled corncob | ND | ND | |
| Milled Soybean | ND | ND | |
| Sugarcane trash | ND | ND | |
| Carbon Supplement (1% w/w) | U·g−1 | % | |
| None | 30.1 ± 1.8 | 100.0 | |
| Peanut hull | 31.9 ± 1.4 | 105.8 | |
| Rice husk | 29.6 ± 1.1 | 98.2 | |
| Soybean meal | 23.7 ± 1.1 | 78.7 | |
| Corn husk | 25.5 ± 1.3 | 84.6 | |
| Sugarcane trash | 30.5 ± 1.7 | 101.3 | |
| Glucose | 28.2 ± 1.1 | 93.7 | |
| Milled corncob | 35.5 ± 1.7 | 117.8 | |
| Nitrogen Supplement | % (w/w) | U·g−1 | % |
| None | -- | 33.5 ± 1.7 | 100.0 |
| Asparagine | 1 | 35.3 ± 1.5 | 105.6 |
| Casein | 1 | 37.3 ± 1.6 | 111.2 |
| Soybean meal | 1 | 26.9 ± 1.2 | 80.4 |
| Glycine | 1 | 29.2 ± 1.3 | 87.4 |
| Peptone | 1 | 46.0 ± 2.4 | 137.4 |
| Yeast extract | 1 | 35.8 ± 1.9 | 107.0 |
| Malt extract | 1 | 39.0 ± 1.9 | 116.4 |
| NH4NO3 | 0.8 | 31.0 ± 1.7 | 92.8 |
| KNO3 | 0.8 | 33.6 ± 1.5 | 100.5 |
| NaNO3 | 0.8 | 17.6 ± 0.9 | 52.2 |
| (NH4)2SO4 | 0.8 | 29.4 ± 1.3 | 87.7 |
| NH4Cl | 0.8 | 56.1 ± 2.7 | 167.8 |
| Urea | 0.8 | 35.9 ± 2.0 | 107.3 |
Pycnoporus sanguineus RP15 was cultured for 192 h at 25 °C in 5 g dry carbon source (or a mass occupying maximally 1/5 of the total volume of the culture flask) and deionized water (2 mL·g−1 dry substrate). Supplementary carbon and nitrogen sources were added to dry wheat bran at the indicated concentrations. The enzymatic assays were performed in duplicate; each experiment was repeated three fold (n = 3). Data are presented as means ± SD. a ND: undetectable by the methods used.