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Purification of Lacps1.
| Step | Total Activity (U) | Total Protein (mg) | Specific Activity (U·mg−1) | Yield (%) | Purification (Fold) |
|---|---|---|---|---|---|
| Crude extract | 548.0 | 32.70 | 16.8 | 100 | 1 |
| 60% (NH4)2SO4 | 344.7 | 13.10 | 26.3 | 63 | 1.6 |
| Phenyl-Sepharose | 164.4 | 0.40 | 411.0 | 30 | 24.5 |
The purification protocol was repeated five-fold. Representative data from one of the purification procedures are presented.
