Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 Apr 1;89(7):3116–3119. doi: 10.1073/pnas.89.7.3116

Stable growth transformation of human T lymphocytes by herpesvirus saimiri.

B Biesinger 1, I Müller-Fleckenstein 1, B Simmer 1, G Lang 1, S Wittmann 1, E Platzer 1, R C Desrosiers 1, B Fleckenstein 1
PMCID: PMC48815  PMID: 1313581

Abstract

Herpesvirus saimiri induces T-cell lymphomas in various species of New World monkeys and in rabbits, and it is able to immortalize monkey T lymphocytes in vitro. Sequences responsible for these effects have been localized to a region of the genome that varies significantly among the virus subgroups A, B, and C. We now report that infection of human blood lymphocytes and thymocytes with strains of subgroup C, in contrast to viruses of the other subgroups, yields continuously proliferating T-cell lines with the phenotype of mature CD4- or CD8-positive cells. Infection with strains of Herpes-virus saimiri subgroup C can thus be used to generate human T-cell lines for a variety of immunological and developmental studies.

Full text

PDF
3117

Images in this article

3117Image
on p.3117
3118Image
on p.3118

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albrecht J. C., Fleckenstein B. Structural organization of the conserved gene block of Herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and major DNA binding protein. Virology. 1990 Feb;174(2):533–542. doi: 10.1016/0042-6822(90)90107-3. [DOI] [PubMed] [Google Scholar]
  2. Alt M., Fleckenstein B., Grassmann R. A pair of selectable herpesvirus vectors for simultaneous gene expression in human lymphoid cells. Gene. 1991 Jun 30;102(2):265–269. doi: 10.1016/0378-1119(91)90088-s. [DOI] [PubMed] [Google Scholar]
  3. Bankier A. T., Dietrich W., Baer R., Barrell B. G., Colbère-Garapin F., Fleckenstein B., Bodemer W. Terminal repetitive sequences in herpesvirus saimiri virion DNA. J Virol. 1985 Jul;55(1):133–139. doi: 10.1128/jvi.55.1.133-139.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Biesinger B., Trimble J. J., Desrosiers R. C., Fleckenstein B. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology. 1990 Jun;176(2):505–514. doi: 10.1016/0042-6822(90)90020-r. [DOI] [PubMed] [Google Scholar]
  5. Bornkamm G. W., Delius H., Fleckenstein B., Werner F. J., Mulder C. Structure of Herpesvirus saimiri genomes: arrangement of heavy and light sequences in the M genome. J Virol. 1976 Jul;19(1):154–161. doi: 10.1128/jvi.19.1.154-161.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daniel M. D., Meléndez L. V., Hunt R. D., King N. W., Anver M., Fraser C. E., Barahona H., Baggs R. B. Herpesvirus saimiri: VII. Induction of malignant lymphoma in New Zealand white rabbits. J Natl Cancer Inst. 1974 Dec;53(6):1803–1807. [PubMed] [Google Scholar]
  7. Desrosiers R. C., Bakker A., Kamine J., Falk L. A., Hunt R. D., King N. W. A region of the Herpesvirus saimiri genome required for oncogenicity. Science. 1985 Apr 12;228(4696):184–187. doi: 10.1126/science.2983431. [DOI] [PubMed] [Google Scholar]
  8. Desrosiers R. C., Falk L. A. Herpesvirus saimiri strain variability. J Virol. 1982 Jul;43(1):352–356. doi: 10.1128/jvi.43.1.352-356.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Desrosiers R. C., Silva D. P., Waldron L. M., Letvin N. L. Nononcogenic deletion mutants of herpesvirus saimiri are defective for in vitro immortalization. J Virol. 1986 Feb;57(2):701–705. doi: 10.1128/jvi.57.2.701-705.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardella T., Medveczky P., Sairenji T., Mulder C. Detection of circular and linear herpesvirus DNA molecules in mammalian cells by gel electrophoresis. J Virol. 1984 Apr;50(1):248–254. doi: 10.1128/jvi.50.1.248-254.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grassmann R., Dengler C., Müller-Fleckenstein I., Fleckenstein B., McGuire K., Dokhelar M. C., Sodroski J. G., Haseltine W. A. Transformation to continuous growth of primary human T lymphocytes by human T-cell leukemia virus type I X-region genes transduced by a Herpesvirus saimiri vector. Proc Natl Acad Sci U S A. 1989 May;86(9):3351–3355. doi: 10.1073/pnas.86.9.3351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grassmann R., Fleckenstein B. Selectable recombinant herpesvirus saimiri is capable of persisting in a human T-cell line. J Virol. 1989 Apr;63(4):1818–1821. doi: 10.1128/jvi.63.4.1818-1821.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jung J. U., Trimble J. J., King N. W., Biesinger B., Fleckenstein B. W., Desrosiers R. C. Identification of transforming genes of subgroup A and C strains of Herpesvirus saimiri. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7051–7055. doi: 10.1073/pnas.88.16.7051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaschka-Dierich C., Werner F. J., Bauer I., Fleckenstein B. Structure of nonintegrated, circular Herpesvirus saimiri and Herpesvirus ateles genomes in tumor cell lines and in vitro-transformed cells. J Virol. 1982 Oct;44(1):295–310. doi: 10.1128/jvi.44.1.295-310.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kiyotaki M., Desrosiers R. C., Letvin N. L. Herpesvirus saimiri strain 11 immortalizes a restricted marmoset T8 lymphocyte subpopulation in vitro. J Exp Med. 1986 Sep 1;164(3):926–931. doi: 10.1084/jem.164.3.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koomey J. M., Mulder C., Burghoff R. L., Fleckenstein B., Desrosiers R. C. Deletion of DNA sequence in a nononcogenic variant of Herpesvirus saimiri. J Virol. 1984 May;50(2):662–665. doi: 10.1128/jvi.50.2.662-665.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lee S. I., Murthy S. C., Trimble J. J., Desrosiers R. C., Steitz J. A. Four novel U RNAs are encoded by a herpesvirus. Cell. 1988 Aug 26;54(5):599–607. doi: 10.1016/s0092-8674(88)80004-7. [DOI] [PubMed] [Google Scholar]
  18. Mantovani A., Allavena P., Sessa C., Bolis G., Mangioni C. Natural killer activity of lymphoid cells isolated from human ascitic ovarian tumors. Int J Cancer. 1980 May 15;25(5):573–582. doi: 10.1002/ijc.2910250505. [DOI] [PubMed] [Google Scholar]
  19. Medveczky M. M., Szomolanyi E., Hesselton R., DeGrand D., Geck P., Medveczky P. G. Herpesvirus saimiri strains from three DNA subgroups have different oncogenic potentials in New Zealand white rabbits. J Virol. 1989 Sep;63(9):3601–3611. doi: 10.1128/jvi.63.9.3601-3611.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Medveczky P., Szomolanyi E., Desrosiers R. C., Mulder C. Classification of herpesvirus saimiri into three groups based on extreme variation in a DNA region required for oncogenicity. J Virol. 1984 Dec;52(3):938–944. doi: 10.1128/jvi.52.3.938-944.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Melendez L. V., Hunt R. D., Daniel M. D., Blake B. J., Garcia F. G. Acute lymphocytic leukemia in owl monkeys inoculated with herpesvirus saimiri. Science. 1971 Mar 19;171(3976):1161–1163. doi: 10.1126/science.171.3976.1161. [DOI] [PubMed] [Google Scholar]
  22. Meléndez L. V., Daniel M. D., García F. G., Fraser C. E., Hunt R. D., King N. W. Herpesvirus saimiri. I. Further characterization studies of a new virus from the squirrel monkey. Lab Anim Care. 1969 Jun;19(3):372–377. [PubMed] [Google Scholar]
  23. Murthy S. C., Trimble J. J., Desrosiers R. C. Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation. J Virol. 1989 Aug;63(8):3307–3314. doi: 10.1128/jvi.63.8.3307-3314.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schirm S., Müller I., Desrosiers R. C., Fleckenstein B. Herpesvirus saimiri DNA in a lymphoid cell line established by in vitro transformation. J Virol. 1984 Mar;49(3):938–946. doi: 10.1128/jvi.49.3.938-946.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simmer B., Alt M., Buckreus I., Berthold S., Fleckenstein B., Platzer E., Grassmann R. Persistence of selectable herpesvirus saimiri in various human haematopoietic and epithelial cell lines. J Gen Virol. 1991 Aug;72(Pt 8):1953–1958. doi: 10.1099/0022-1317-72-8-1953. [DOI] [PubMed] [Google Scholar]
  26. Trimble J. J., Murthy S. C., Bakker A., Grassmann R., Desrosiers R. C. A gene for dihydrofolate reductase in a herpesvirus. Science. 1988 Mar 4;239(4844):1145–1147. doi: 10.1126/science.2830673. [DOI] [PubMed] [Google Scholar]
  27. Valerius T., Repp R., Kalden J. R., Platzer E. Effects of IFN on human eosinophils in comparison with other cytokines. A novel class of eosinophil activators with delayed onset of action. J Immunol. 1990 Nov 1;145(9):2950–2958. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES