Table 1.
Quantification (mean ± standard deviation (SD), n = 3) by HPLC-DAD-ESI-QTOF-MS in negative and positive mode of individual compound tentatively identified in P. guajava leaves for the different oxidative states.
| No. | Compound | High | Medium | Low |
|---|---|---|---|---|
| Negative mode | Concentration (μg compound/g leaf d.w.) | |||
| 1 | HHDP glucose Isomer | 526 ± 2 c | 651 ± 19 b | 936 ± 10 a |
| 2 | HHDP glucose Isomer | 505 ± 3 c | 645 ± 3 b | 823 ± 16 a |
| 3 | HHDP glucose Isomer | 510 ± 11 c | 645 ± 20 b | 934 ± 2 a |
| 4 | Prodelphinidin B Isomer | 447.1 ± 0.1 c | 515.7 ± 0.4 b | 715 ± 13 a |
| 5 | Gallic acid | 153.52 ± 0.09 c | 164 ± 3 b | 175.9 ± 0.7 a |
| 6 | Pedunculagin/Casuariin Isomer | 158.8 ± 0.6 b | 163.84 ± 0.06 b | 175 ± 3 a |
| 7 | Pedunculagin/Casuariin Isomer | 464.0 ± 0.8 c | 475.5 ± 0.5 b | 557 ± 2 a |
| 8 | Prodelphinidin Dimer Isomer | 497 ± 1 b | 529 ± 6 b | 603 ± 30 a |
| 9 | Gallocatechin | 4913 ± 47 a | 4435 ± 7 b | 4098 ± 84 c |
| 10 | Vescalagin/castalagin Isomer | 157.59 ± 0.01 a | 136.6 ± 0.3 c | 143 ± 2 b |
| 11 | Prodelphinidin Dimer Isomer | 1365 ± 7 c | 1560 ± 14 b | 1739 ± 25 a |
| 12 | Uralenneoside | 2464 ± 4 a | 1911 ± 24 b | 1872 ± 81 b |
| 13 | Geraniin Isomer | 241 ± 1 b | 264.9 ± 0.5 b | 343 ± 25 a |
| 14 | Pedunculagin/Casuariin Isomer | 466 ± 3 c | 575 ± 16 b | 683 ± 20 a |
| 15 | Geraniin Isomer | 260 ± 3 b | 290 ± 7 a,b | 356 ± 48 a |
| 16 | Procyanidin B Isomer | 4262 ± 12 c | 4742 ± 15 b | 5514 ± 69 a |
| 17 | Galloyl(epi)catechin-(epi)gallocatechin | <LOQ | 12.60 ± 0.07 b | 38 ± 3 a |
| 18 | Procyanidin B Isomer | 650 ± 3 c | 708 ± 11 b | 757 ± 23 a |
| 19 | Tellimagrandin I Isomer | 347 ± 4 c | 367.2 ± 0.7 b | 397 ± 2 a |
| 20 | Pterocarinin A Isomer | 569 ± 31 b | 617 ± 9 b | 679 ± 7 a |
| 21 | Pterocarinin A Isomer | 316 ± 2 c | 360 ± 4 b | 376 ± 4 a |
| 22 | Stenophyllanin A | 853 ± 13 c | 1036 ± 50 b | 1318 ± 24 a |
| 23 | Procyanidin trimer Isomer | 781 ± 1 a | 706 ± 1 c | 738 ± 4 b |
| 24 | Catechin | 8486 ± 10 b | 8957 ± 11 a | 6845 ± 24 c |
| 25 | Procyanidin tetramer | <LOQ | <LOQ | <LOQ |
| 26 | Procyanidin trimer Isomer | 89 ± 2 c | 108 ± 3 b | 128 ± 1 a |
| 27 | Guavin A | 263 ± 9 c | 357 ± 8 b | 518 ± 15 a |
| 28 | Casuarinin/Casuarictin Isomer | 1297 ± 5 c | 1568 ± 10 b | 2089 ± 11 a |
| 29 | Galloyl(epi)catechin-(epi)gallocatechin | 61 ± 5 c | 135 ± 1 b | 211 ± 12 a |
| 30 | Procyanidin pentamer | <LOQ | <LOQ | <LOQ |
| 31 | Galloyl-(epi)catechin trimer Isomer | <LOQ | <LOQ | <LOQ |
| 32 | Gallocatechin | 2074 ± 2 b | 1526 ± 2 c | 2613 ± 55 a |
| 33 | Tellimagrandin I Isomer | 463 ± 2 c | 516 ± 6 b | 737 ± 24 a |
| 34 | Vescalagin | 160 ± 6 b | 159 ± 3 b | 187 ± 1 a |
| 35 | Stenophyllanin A Isomer | 355.36 ± 0.07 c | 425 ± 13 b | 548 ± 20 a |
| 36 | Galloyl-(epi)catechin trimer Isomer | <LOQ | <LOQ | <LOQ |
| 37 | Myricetin hexoside Isomer | 432.10 ± 0.05 c | 555 ± 2 b | 572 ± 7 a |
| 38 | Stachyuranin A | 207.40 ± 0.04 a | 207 ± 6 a | 216 ± 1 a |
| 39 | Procyanidin gallate Isomer | 533.2 ± 0.07 c | 799 ± 3 b | 1036 ± 32 a |
| 40 | Myricetin hexoside Isomer | 213 ± 2 c | 288 ± 2 b | 307.8 ± 0.8 a |
| 41 | Vescalagin/castalagin Isomer | 152 ± 3 b | 155 ± 2 b | 191 ± 3 a |
| 42 | Myricetin arabinoside/xylopyranoside Isomer | 241 ± 5 c | 286 ± 2 b | 306 ± 5 a |
| 43 | Myricetin arabinoside/xylopyranoside Isomer | 608 ± 1 c | 839 ± 8 b | 946 ± 11 a |
| 44 | Procyanidin gallate Isomer | 11 ± 1 a | 3.7 ± 0.2 b | <LOQ |
| 45 | Myricetin arabinoside/xylopyranoside Isomer | 688 ± 16 c | 816.0 ± 0.5 b | 874 ± 9 a |
| 46 | Myricetin hexoside Isomer | 1186 ± 13 a | 1010 ± 3 b | 1012 ± 65 b |
| 47 | Myricetin hexoside Isomer | 200 ± 3 b | 208 ± 5 b | 224 ± 6 a |
| 48 | Myricetin arabinoside/xylopyranoside Isomer | 276.0 ± 0.9 a,b | 266 ± 3 b | 282 ± 8 a |
| 49 | Quercetin galloylhexoside Isomer | 375.0 ± 0.6 b | 380 ± 5 b | 438 ± 18 a |
| 50 | Ellagic acid deoxyhexoside | 700 ± 1 a | 702 ± 12 a | 733 ± 32 a |
| 51 | Quercetin galloylhexoside Isomer | 180 ± 2 b | 194 ± 7 a | 205 ± 1 a |
| 52 | Myricetin arabinoside/xylopyranoside Isomer | 544.3 ± 0.4 b | 525 ± 2 b | 588 ± 18 a |
| 53 | Morin | 2619 ± 4 c | 3206 ± 11 b | 4474 ± 98 a |
| 54 | Myricetin arabinoside/xylopyranoside Isomer | 611 ± 4 a | 581 ± 6 b | 559 ± 3 c |
| 55 | Ellagic acid | 1229 ± 26 c | 1345 ± 34 b | 1759.6 ± 0.9 a |
| 56 | Hyperin | 11305 ± 27 c | 11906 ± 57 b | 12528 ± 83 a |
| 57 | Quercetin glucuronide | <LOQ | <LOQ | <LOQ |
| 58 | Isoquercitrin | 2254 ± 10 b | 2471 ± 16 b | 3410 ± 38 a |
| 59 | Procyanidin gallate Isomer | <LOQ | 7.3 ± 0.3 b | 73.3 ± 0.2 a |
| 60 | Reynoutrin | 2641 ± 11 b | 2762 ± 2 b | 3210 ± 104 a |
| 61 | Guajaverin | 8864 ± 8 b | 9668 ± 64 b | 11813 ± 64 a |
| 62 | Guavinoside A | 783 ± 5 a,b | 770 ± 4 b | 793 ± 4 a |
| 63 | Avicularin | 10353 ± 18 a,b | 10173 ± 54 b | 11441 ± 63 a |
| 64 | Quercitrin | 213 ± 2 b | 208 ± 2 b | 223 ± 1 a |
| 65 | Myrciaphenone B | 546 ± 6 c | 621 ± 1 b | 715 ± 20 a |
| 66 | Guavinoside C | 2069 ± 1 b | 1966 ± 21 c | 2209 ± 21 a |
| 67 | Guavinoside B | 872 ± 17 c | 1035 ± 23 b | 1273 ± 30 a |
| 68 | Guavinoside A Isomer | 137 ± 1 a | 135.1 ± 0.6 a | 137 ± 3 a |
| 69 | Guavinoside B Isomer | 120 ± 2 b | 119,6 ± 0.2 b | 129 ± 1 a |
| 70 | 2,6-dihydroxy-3-methyl-4-O-(6″-O-galloyl-β-d-glucopyranosyl)-benzophenone | 1179 ± 12 b | 1242 ± 33 b | 1365 ± 20 a |
| 71 | Guavin B | 220.51 ± 0.03 b | 230.1 ± 0.7 a,b | 241 ± 7 a |
| 72 | Quercetin | 258 ± 4 a | 253 ± 3 a | 255 ± 5 a |
| 73 | Naringenin | 487 ± 3 c | 638 ± 24 b | 705 ± 6 a |
| Positive mode | Concentration (μg compound/g leaf d.w.) | |||
| 74 | Cyanidin-3-O-glucoside | 441.28 ± 0.04 a | 169.3 ± 0.5 b | 29.5 ± 0.2 c |
LOQ: limits of quantification; means in the same line with different letter (a,b,c) are significantly different (p < 0.05).