Table 2.
Effect of HB12 and TT8 down-regulation on carbohydrate subfractions in model forage (Medicago sativa spp. Sativa: Alfalfa) a.
| Items | Non-Transgenic (NT) | Gene silencing through RNAi Technology (GS) | SEM b | p Value | Contrast, p Value | |
|---|---|---|---|---|---|---|
| Control | HB12 | TT8 | NT vs. GS | |||
| Fractions of Carbohydrate Partitioned by CNCPS c (% CHO) | ||||||
| CA4 | 7.37 | 4.18 | 4.41 | 0.867 | 0.135 | 0.063 |
| CB1 | 5.26 a | 2.04 b | 2.58 b | 0.334 | 0.012 | 0.006 |
| CB2 | 49.35 | 49.79 | 49.41 | 1.201 | 0.962 | 0.876 |
| CB3 | 29.89 | 29.64 | 31.30 | 1.534 | 0.735 | 0.779 |
| CC | 8.14 | 14.36 | 12.31 | 1.908 | 0.209 | 0.113 |
a means with different letters within the same row differ significantly (p < 0.05); b SEM: stand error of the mean; The Tukey-Kramer method was used for multi-treatment comparison; c CNCPS: Cornell Net Carbohydrate and Protein System; Carbohydrate CNCPS fractions undetected in alfalfa populations include: CA1 fraction (volatile fatty acids), CA2 fraction (lactate acid), CA3 (other organic acids). Measurable CNCPS alfalfa carbohydrate fractions include: CA4 (sugar), CB1 (starch), CB2 (soluble fiber), CB3 (available NDF) and CC (indigestible fiber).