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. 2016 May 14;17(5):738. doi: 10.3390/ijms17050738

Figure 5.

Figure 5

(A) Cells were double-stained with Annexin V-FITC/PI and apoptosis rates was analyzed using flow cytometry (p < 0.05 as compared with control); (B) protein levels of cleaved caspase 3, Bax, and Bcl2 were detected by Western blot. Data were shown as mean ± SD (p < 0.05 as compared with control). The mean of targeted protein/β-actin expression in PC-3 cells set as 1.0.Control: wild-type cells; AQP9-siRNA: cells transfected with AQP9 specific small interfering RNA; Mock: cells only treated with Lipofectamine 2000. * p < 0.05; ** p < 0.01.