. 2016 May 18;17(5):749. doi: 10.3390/ijms17050749

Table 1.

Summary of reported miRNAs and their targets in cardiac hypertrophy.

miRNAs	Targets		miRNA–mRNA Interaction		Platforms Remarks	References
miRNAs	mRNA	Signaling Pathway	Luciferase Assay	Gain/Loss of Function	Platforms Remarks	References
Anti-hypertrophic
miR-1	Fbln2	ECM	+	+	AAB rat	[11]
	Twf1	ECM	+	+	NRCMs	[10]
	Igf1	PI3K-Akt	+	+	TAC mouse and AAC rat	[43,44]
	Fabp3	PPAR	+	+	NMCMs and TAC mouse	[45]
miR-101	Rab1a	MAPKK	+	+	TAAC rat	[12]
miR-133	RhoA	cGMP-PKG	+	+	NMCMs and TAC mouse	[13]
	Cdc42	MAPK	+	+	NMCMs and TAC mouse
	Nelfa/Whsc2	Transcription	+	+	NMCMs and TAC mouse
miR-145	GATA6	cGMP-PKG	+	+	NRCMs and TAC mouse	[14]
miR-150	p300	FoxO	+	+	NRCMs	[15]
miR-185	Camk2d	Calcium	+	+	NRVMs and TAC mouse	[16]
	Ncx1	cGMP-PKG	+	+	NRVMs and TAC mouse
	Nfatc3	cGMP-PKG	+	+	NRVMs and TAC mouse
miR-223	Tni3k		+	+	NRCMs and TAC mouse	[46]
miR-26b	GATA4	cGMP-PKG	+	+	NRCMs and TAC mouse	[18]
miR-30-3p	Xbp1	VEGF	+	+	H9c2 cells and AAC rat	[40]
miR-34a	Atg9a	Autophagy	+	−	NRCMs and TAAC rat	[19]
miR-378	Mapk1	MAPK	+	+	NRCMs and TAC mouse	[20]
	Igf1r	MAPK	+	+	NRCMs and TAC mouse
	Grb2	MAPK	+	+	NRCMs and TAC mouse
	Ksr1	MAPK	+	+	NRCMs and TAC mouse
miR-9	Myocardin	cGMP-PKG	+	−	NRCMs	[21]
miR-98	Cyclin D2	PI3K-AKT	+	−	NRCMs	[22]
Pro-hypertrophic
miR-155	Tp53inp1	p53	+	+	NMCFs and AMI mouse	[23]
miR-199a	Gsk3β	PI3K-AKT	+	+	NRCMs and TG mouse	[25]
miR-199b	Dyrk1a	calcineurin/NFAT	+	+	NRCMs and TAC mouse	[27]
miR-19a/b	Atrogin1	calcineurin/NFAT	+	+	NRCMs and TAC mouse	[24]
miR-19a/b	Murf1	PKC	+	+	NRCMs and TAC mouse	[24]
miR-208a	Thrap1	Thyroid hormone	+	+	NRCMs, TAC and TG mouse	[28]
miR-208a	Myostatin	Cell growth/Differentation	+	+	NRCMs, TAC and TG mouse	[28]
miR-21	Pten	PI3K-AKT	+	−	Human glomerular mesangial cells	[47]
miR-21-3p	Hdac8	AKT/GSK3β	+	+	TAC mouse	[30]
miR-212/132 family	Foxo3	PI3K-Akt	+	+	H9c2 cells and TAC mouse	[32]
miR-22	Sirt1	AMPK	+	+	NRCMs, miR-22 KO mouse	[36]
	Hdac4	AMPK	+	+	NRCMs, miR-22 KO mouse	[36]
	Pten	PI3K-AKT	+	+	NRCMs	[35]
miR-221	p27	PI3K-AKT	+	+	NRCM, TAC mouse	[17]
miR-23a	Foxo3a	PI3K-AKT	+	−	NMCMs, TAC and TG mouse	[33]
miR-23a	Lpa1	PI3K-AKT	+	+	NRCMs	[34]
miR-27b	Pparγ	PPAR	+	+	NRCMs, TAC and TG mouse	[38]
miR-30a	Beclin 1	Autophagy	+	+	NRCMs, TAAC rat	[39]
miR-328	Serca2a	cGMP-PKG	+	+	NRVCs, TAC and TG mouse	[41]
miR-350	Mapk11/14	MAPK	+	+	H9c2 cells and TAC rats	[42]
miR-350	Mapk8/9	MAPK	+	+	H9c2 cells and TAC rats	[42]

Abbreviations for mRNAs: Fbln2, Fibulin 2; Twf1, Twinfilin 1; Igf1, Insulin-like growth factor 1; Fabp3, Fatty Acid Binding Protein 3; Rab1a, Ras-related protein Rab 1a; Rhoa, Ras homolog family member A; Cdc42, Cell Division Cycle 42; Nelfa/Whsc2, Wolf-Hirschhorn syndrome candidate 2 protein; GATA6, GATA binding protein 6; p300, E1A binding protein p300; Camk2d, calcium/calmodulin-dependent protein kinase II delta; Ncx1, sodium/calcium exchanger 1; Nfatc3, Nuclear factor of activated T-cells, cytoplasmic, calcineurin dependent 3; Tnni3k, Troponin I type 3 interacting kinase; GATA4, GATA binding protein 4; Xbp1, X-box binding protein 1; Atg9a, autophagy-related 9A; Mapk1, mitogen-activated protein kinase 1; Igf1r, insulin-like growth factor 1 receptor; Grb2, Growth factor receptor bound protein 2; Ksr1, kinase suppressor of ras 1; Tp53inp1, tumor protein p53 inducible nuclear protein 1; Gsk3β, glycogen synthase kinase 3 β; Dyrk1a, Dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a; Murf1, tripartite motif-containing 63; Thrap1, Mediator complex subunit 13; Pten, phosphatase and tensin homolog; Hdac8, histone deacetylase 8; Foxo3, Forkhead box O3; Sirt1, Sirtuin 1; Hdac4, histone deacetylase 4; Foxo3a, Forkhead box O3A; Lpa1, Lysophosphatidic acid receptor 1; Pparγ, peroxisome proliferator-activated receptor γ; Serca2a, Sarco/endoplasmic reticulum Ca²⁺-ATPase 2a; Mapk11/14, mitogen-activated protein kinase 11/14; Mapk8/9, Mitogen-activated protein kinase 8/9. Abbreviations for pathway: ECM, extracellular matrix deposition; PI3K-Akt, phosphatidylinositol 3 kinase-protein kinase B; PPAR, peroxisome proliferator-activated receptor; cGMP-PKG, cyclic guanosine monophosphate-dependent protein kinase G; VEGF, vascular endothelial growth factor; Akt-Gsk3β, protein kinase B-Glycogen synthase kinase 3 β; FoxO, Forkhead box; MAPK, mitogen-activated protein kinase; p53, tumor protein p53; NFAT, nuclear factor of activated T-cells; PKC, protein kinase C; AMPK, AMP-activated protein kinase. Abbreviations for platforms: AAB rat, abdominal aortic banding rat; NRCMs, neonatal rat cardiomyocytes; TAC mouse, transverse aortic constriction mouse; ACC mouse, abdominal aortic constriction mouse; NMCMs, neonatal mouse cardiomyocytes; TAAC rat, transverse abdominal aortic constriction; NRVMs, neonatal rat ventricular myocytes; H9c2 cells, rat myoblast cells; NMCFs, Neonatal mouse cardiac fibroblasts; AMI mouse, acute myocardial infarction mouse; TG mouse, transgenic mouse; KO mouse, knockout mouse; NRVCs, neonatal rat ventricular cells. +/− indicates with or without experimental results to support the regulatory effect of microRNAs on target gene expressions respectively.