Figure 3.

Thermostability assays of recombinant Wild Type glucose-6-phosphate dehydrogenase (WT G6PD) and the three mutants with different NADP⁺ concentrations: (A) WT G6PD; (B) G6PD Zacatecas (R257L); (C) G6PD Vanua-Lava (L128P); and (D) G6PD Viangchan (V291M). In all cases, 200 ng of total protein was used. Residual activity was expressed as a percentage of the activity for the same sample incubated at 37 °C. The assays were performed in duplicate; standard errors were lower than 5%. NADP⁺ concentrations: without NADP⁺ (○), 10 µM (•), 100 µM (∆) and 500 µM (▼) NADP⁺, respectively.