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. 2016 May 21;17(5):787. doi: 10.3390/ijms17050787

Figure 7.

Figure 7

Spectroscopic characterization of recombinant human G6PD enzymes. Far-UV CD spectra of WT G6PD and mutants without (A) or with (B) 0.25 M Gdn-HCl were performed in spectropolarimeter (Jasco J-810®) equipped with a Peltier thermostatted cell holder; standard errors were less than 4%. In all cases, the protein concentration was 0.2 mg/mL and incubated by 2 h at 37 °C and after than measured by CD. For both trials, the spectra of blanks were subtracted from those that contained the protein.