Table 2.

Catalytic properties of human G6PD and the mutants.

Kinetic Constants	WT-G6PD	Mutants
		Zacatecas	Vanua-Lava	Viangchan
KmG6P (µM)	38.49	111	34	42
KmNADP+ (µM)	6.16	24	18	17
kcat (s−1)	230	58	142	145
kcat/Km·G6P (s−1·M−1)	5.97 × 106	0.52 × 106	4.17 × 106	3.45 × 106
kcat/KmNADP+ (s−1·M−1)	37.33 × 106	2.41 × 106	7.88 × 106	8.52 × 106

The kinetic constants estimated for the WT G6PD and the Class I G6PD Zacatecas and two Classes II G6PD Vanua-Lava and Viangchan mutants. The parameters in each case were obtained from three independent experiments and from different enzyme preparations.