Table 1.

RPA primers used to detect EGFR 19Del(2) and L858R mutations.

EGFR Mutation Type Primers Amplicon (bp)
19Del(2)FP 5’-GTGAGAAAGTTAAAATTCCCGTCGCTATCAAGACATCTC-3’ 266
19Del(2)RP 5’-GATACCAGCATGGGAGAGGCCAGTGCTGTCTCTAAG-3’
L858R FP 5’-CTGAATTCGGATGCAGAGCTTCTTCCCATGA-3’ 201
L858R RP 5’-CTTTCTCTTCCGCACCCAGCAGTTTGGCCC-3’
PNA 5’-GCCAGCCCAA-3’

FP, Forward Primer; RP, Reverse Primer; PNA, peptide nucleic acid.