Table 2.

PCR primers used to detect EGFR mutations

EGFR Mutation Type Primers Amplicon (bp)
19Del FP 5’-AACGTCTTCCTTCTCTCTCTGTCA-3’ 135(Mut)/150(Wild)
19Del RP 5’-CCACACAGCAAAGCAGAAACT-3’
L858R FP 5’-CTGAATTCGGATGCAGAGCTT-3’ 291
L858R RP 5’-CTAGTGGGAAGGCAGCCTGGT-3’

FP: Forward Primer, RP: Reverse Primer, Mut: mutation type, Wild: wild type.