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Neurogenesis. Three weeks following transplantation of MSC, MSC/BDNF MOI 10, or vehicle control (Normosol-R), the 10-week-old R6/2 mice were euthanized and formalin perfused. Brains were cryosectioned at 30 μm and labeled using doublecortin and AlexaFluor488. The subventricluar zone was imaged using a Zeiss Axioskop 2 microscope (10× objective) and average fluorescent intensity was calculated using ImageJ. * = Significant to WT, # = Significant to R6/2 + Normosol. n = 6–7/group.
