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. 2016 Mar 15;24(5):915–925. doi: 10.1038/mt.2016.36

Figure 3.

Figure 3

Loss of Chop attenuates TGF-β signalling after BLM induction. (a) Western blot analysis of TGF-β1 expression in the lung homogenates. Left panel: representative western blot results. Right panel: a bar graph showing the data of all mice studied. (b) ELISA results for TGF-β1 levels in the BALF. (c) Results for western blot analysis of TGF-β downstream Smad2 and 3 activities. Left panel: a representative western blot result for Smad2/3, p-smad2, and p-smad3 in the lung homogenates. Right panel: a bar graph showing the results of all mice examined. (d) Coimmunostaining of TGF-β1 and F4/80 in the lung sections. The images were taken under original magnification ×400. Each bar represents the mean ± SEM of 12 mice studied. **P < 0.01; ***P < 0.001. BALF, bronchoalveolar lavage fluids; BLM, bleomycin; TGF-β, transforming growth factor β.