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. 2016 Mar 22;24(5):946–955. doi: 10.1038/mt.2016.39

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

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Single-stranded and double-stranded miR-34a mimics reduce mRNA levels of miR-34a target genes. (a) Quantitative polymerase chain reaction (qPCR) data showing the effect of modified ss-mR-34a-5p, ss-miR-34a 2'-F(GU), ss-miR-34a 2'-F(AA), and unmodified ds-miR-34a(fc) (50 nmol/l) on expression of miR-34a target genes relative to ss-Ctrl1 treatment. Cells were harvested 2 days after transfection for qPCR analysis. Error bars are mean ± SD. n = 4. Complete qPCR data for 20 potential miR-34a target genes are presented in Supplementary Figure S2. (b) Dose response profiles for ARHGAP1, E2F5, and CDK6 inhibition by ss- or ds-miR-34a mimics. Cells were harvested 2 days after transfection for qPCR analysis. Error bars are mean ± SD. n = 4. (c) qPCR data showing expression of genes that lack target sites for miR-34a after treating HeLa cells with miR-34a mimics (50 nmol/l). Cells were harvested 2 days after transfection for qPCR analysis. Error bars are mean ± SD. n = 4.